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The introduction of the cell tradition technique within the 1980s, by which disparate cell varieties may cohabit in a petri dish bathed in a supportive medium, proved necessary. The co-culturing of neurones and Healthy Flow Blood glia led to the first essential discovery, particularly that neurones survive higher in culture when astrocytes are also current (Whatley et al., 1981). This preliminary research did not reveal what aspect of the presence of astrocytes the neurones discovered essential for survival. This was not a trivial matter since the assist afforded by astrocytes may very well be physical, where connections between cells are important, or the release of some trophic issue from astrocytes that supported the neurones. A later culture examine revealed that the essential part of astrocyte presence that supported neurones was the glycogen contained throughout the astrocytes (Swanson, 1992; Swanson and Choi, 1993). Astrocytes depleted of glycogen were not as successful at supporting neurones as astrocytes with a full complement of glycogen.

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